2014—2015年豫南地区猪流行性腹泻病毒S基因变异分析

doi:10.11843/j.issn.0366-6964.2018.04.026

Abstract

Abstract:

To investigate the variation of PEDV S gene in Henan province, specific primers were designed and synthesized, S genes of 38 PEDV strains from different pig farms in Henan provinces from 2014-2015 were amplified by RT-PCR, then cloned and sequenced. The phylogenetic relationships and protein characterization of the full-length PEDV S protein in Henan of China were analyzed with other PEDV reference strains. Phylogenetic analysis indicated that all the amplified S genes had a close relationship with some variant strains isolated from China and USA in recent years. Sequence alignment analysis showed that extensive amino acid (AA) insertion and deletion were found in the S protein. Furthermore, there were 11 AA deletion at the C terminal of S protein encoded by amplicated S gene. Many strains had wide AA substitution in the neutralizing epitopes COE and 2C10. These results will provide valuable research basis for understanding the variant characterization of PEDV and controlling the outbreak of PED in this area.

CLC Number:

S852.659.6

DONG Jian-guo, WANG Rui, QU Zhe-hui, ZHAO Yu, LIU Tao. Genetic Variations of S Gene of Porcine Epidemic Diarrhea Virus in Sourthern Henan Province from 2014 to 2015[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 859-864.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2018.04.026

https://www.xmsyxb.com/EN/Y2018/V49/I4/859

References

[1] 殷震, 刘景华. 动物病毒学[M]. 2版. 北京:科学出版社, 1997:681-688.
YIN Z, LIU J H. Animal virology[M]. 2nd ed. Beijing:Science Press, 1997:681-688. (in Chinese)
[2] BRIDGEN A, DUARTE M, TOBLER K, et al. Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus[J]. J Gen Virol, 1993,74(Pt 9):1795-1804.
[3] CHANG S H, BAE J, KANG T J, et al. Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus[J]. Mol Cells, 2002, 14(2):295-299.
[4] CHEN J F, WANG C B, SHI H Y, et al. Molecular epidemiology of porcine epidemic diarrhea virus in China[J]. Arch Virol, 2010, 155(9):1471-1476.
[5] CHEN X, YANG J X, YU F S, et al. Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) samples from field cases in Fujian, China[J]. Virus Genes, 2012, 45(3):499-507.
[6] 张强敏, 郭福生, 尹燕博, 等. 猪流行性腹泻病毒分子生物学特征[J]. 中国病毒学, 2002, 17(4):381-384.
ZHANG Q M, GUO F S, YIN Y B, et al. Molecular biological traits of Porcine epidemic diarrhea virus[J]. Virologica Sinica, 2002, 17(4):381-384. (in Chinese)
[7] GAO Y Y, KOU Q W, GE X N, et al. Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China[J]. Arch Virol, 2013, 158(3):711-715.
[8] GE J W, LIU D Q, LI Y J. Construction of recombinant lactobacilli expressing the core neutralizing epitope (COE) of porcine epidemic diarrhea virus and a fusion protein consisting of COE and Escherichia coli heat-labile enterotoxin B, and comparison of the immune responses by orogastric immunization[J]. Can J Microbiol, 2012, 58(11):1258-1267.
[9] HUANG Y W, DICKERMAN A W, PIÑEYRO P, et al. Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States[J]. mBio, 2013, 4(5):e00737-13.
[10] 刘孝珍, 陈建飞, 时洪艳, 等. 2011年猪流行性腹泻病毒的遗传变异分析[J]. 中国预防兽医学报, 2012, 34(3):180-183.
LIU X Z, CHEN J F, SHI H Y, et al. Genetic variation analysis of porcine epidemic diarrhea virus isolated in 2011[J]. Chinese Journal of Preventive Veterinary Medicine, 2012, 34(3):180-183. (in Chinese)
[11] LEE D K, PARK C K, KIM S H, et al. Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea[J]. Virus Res, 2010, 149(2):175-182.
[12] MARTHALER D, JIANG Y, OTTERSON T, et al. Complete genome sequence of porcine epidemic diarrhea virus strain USA/Colorado/2013 from the United States[J]. Genome Announc, 2013, 1(4):e00555-13.
[13] PARK S J, MOON H J, YANG J S, et al. Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea[J]. Virus Genes, 2007, 35(2):321-332.
[14] PENSAERT M B, DE BOUCK P. A new coronavirus-like particle associated with diarrhea in swine[J]. Arch Virol, 1978, 58(3):243-247.
[15] SONG D, PARK B. Porcine epidemic diarrhoea virus:a comprehensive review of molecular epidemiology, diagnosis, and vaccines[J]. Virus Genes, 2012, 44(2):167-175.
[16] SPAAN W, CAVANAGH D, HORZINEK M C. Coronaviruses:structure and genome expression[J]. J Gen Virol, 1988, 69(Pt 12):2939-2952.
[17] SUN D B, FENG L, SHI H Y, et al. Identification of two novel B cell epitopes on porcine epidemic diarrhea virus spike protein[J]. Vet Microbiol, 2008, 131(1-2):73-81.
[18] SUN R Q, CAI R J, CHEN Y Q, et al. Outbreak of porcine epidemic diarrhea in suckling piglets, China[J]. Emerg Infect Dis, 2012, 18(1):161-163.
[19] TEMEEYASEN G, SRIJANGWAD A, TRIPIPAT T, et al. Genetic diversity of ORF3 and spike genes of porcine epidemic diarrhea virus in Thailand[J]. Infect Genet Evol, 2014, 21:205-213.
[20] WANG X M, NIU B B, YAN H, et al. Complete genome sequence of a variant porcine epidemic diarrhea virus strain isolated in central China[J]. Genome Announc, 2013, 1(1):e00243-12.

Genetic Variations of S Gene of Porcine Epidemic Diarrhea Virus in Sourthern Henan Province from 2014 to 2015

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SONG Wenyan, ZHANG Hanwen, WU Aodi, ZHANG Liyan, LIU Zhao, YE Tongtong, CHEN Chuangfu, SHENG Jinliang. Screening of Nanobodies against Porcine Reproductive and Respiratory Syndrome Virus GP5 Protein and Exploration of Their Inhibitory Effect on Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 258-270.
[2]	WANG Ying, LI Jiakang, ZENG Yue, SHI Kaituo, WEI Liting, PENG Jiajia, LI Qiuyan, CAO Longlong, ZHOU Dengyuan. Development of Monoclonal Antibodies against Feline Calicivirus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 401-405.
[3]	LI Huihui, DONG Keer, LIU Xinbo, ZHANG Chunxiao, MA Chao, CHEN Liping, ZHONG Qi, YAO Gang, MA Xuelian. Establishment and Application of Rapid Detection Method for Bovine Norovirus and Bovine Rotavirus Dual RAA-LFD [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 406-412.
[4]	ZHOU Jianhao, WANG Dongfang, LIU Ying, WANG Shujuan, MA Zhenyuan, XIE Caihua, ZHAO Xueli, YANG Haibo, FENG Guidan, KANG Taisheng, HU Yufeng, LI Bowen, YAN Ruoqian. Establishment and Preliminary Application of a Quantitative Droplet Digital PCR Assay for Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 413-418.
[5]	ZHANG Jiaqi, JIA Xining, ZHOU Qun, SONG Xin, ZHU Chenxi, LI Gege, ZHANG Bin. Construction of BVDV-1 Virus-like Particles based on E2 Protein and Evaluation of Its Immune Efficacy in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5143-5153.
[6]	ZHOU Likun, DONG Xinyuan, LI Jiahui, CUI Zhiying, ZHAO Shijie, XU Jie, CHEN Jing, ZHANG Yina, XIA Ping’an. Regulatory Role of MID2 on Porcine Reproductive and Respiratory Syndrome Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4735-4744.
[7]	ZHENG Ruwen, HUANG Tao, WU Daoyi, YU Guangmei, LI Fangfang, SUI Xin, MIN Tingyu. Establishment and Preliminary Application of LAMP-LFD Detection Method for Bovine Viral Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4745-4753.
[8]	JIA Yiquan, YU Mengting, LIU Weirong, FANG Shouguo, ZHANG Songbai. Baicalin Inhibits Infectious Bronchitis Virus Replication via Promoting the Expression of Type Ⅰ Interferon [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4839-4850.
[9]	CHEN Lu, CHANG Xinyu, SHEN Jiachen, SHEN Ruiting, ZHAO Zhenhua, HOU Xiaolin. Transcriptome Sequencing and Analysis of HD11 Cells Infected by Avian Infectious Bronchitis Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4860-4865.
[10]	HE Yeqing, ZUO Baijiao, LI Qinghao, SUN Juan, JIN Xin, XIA Lu, WEI Zhanyong. Histopathological Observation of Immune Organs of Piglets Infected with Transmissible Gastroenteritis Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4872-4879.
[11]	CHEN Wenzhe, ZHANG Xiangle, GU Fengxing, ZHAO Zhenxiang, LI Kangli, XUE Zhaoning, ZHENG Haixue, ZHANG Xiaoli, ZHU Zixiang. Evaluation of the Antiviral Effect of Porcine GRK2 against Foot-and-mouth Disease Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4350-4361.
[12]	ZOU Hong, XIA Yingju, LI Ling, XU Lu, ZHAO Junjie, WANG Tuanjie, ZHANG Qianyi, SONG Zhenhui. Establishment and Preliminary Application of a Dual Real-time RT-PCR Assay for CSFV and BVDV [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4422-4427.
[13]	YUAN Li, SUN Yangyang, ZHANG Lujie, ZHANG Jie, SUN Haifeng, BAI Juan, JIANG Ping. Production and Epitope Characterization of Monoclonal Antibodies against GP3 Protein of Porcine Reproductive and Respiratory Syndrome Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3424-3434.
[14]	CAO Liyan, KONG Xiangyu, LI Xiangtong, SUO Xuepeng, DUAN Yueyue, YUAN Cong, SHI Lei, ZHANG Yu, MA Guoxiang, ZHENG Haixue, WANG Qi. Preparation and Sequence Analysis of Monoclonal Antibody against the Nucleocapsid Protein of Porcine Acute Diarrhea Syndrome Coronavirus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2487-2497.
[15]	ZHANG Senhao, WANG Xueying, CAI Limeng, XIE Weichun, KUANG Hongdi, WANG Xiaona, LI Jiaxuan, CUI Wen, JIANG Yanping, ZHOU Han, SHAN Zhifu, WANG Li, QIAO Xinyuan, LI Yijing, TANG Lijie. Establishment and Application of Rapid Diagnosis Method for RPA of Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2498-2508.